Disk diffusion, also known as Kirby-Bauer antibiotic testing, involves application of commercially available drug-impregnated filter paper disks to the surface of an agar plate that has been inoculated to confluence with the organism of interest.Click to see full answer. Then, what is the Kirby Bauer disc diffusion method?The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic susceptibility test in determining what choice of antibiotics should be used when treating an infection. This method relies on the inhibition of bacterial growth measured under standard conditions.Similarly, what is Stoke method? The stokes’ method allows each individual isolate to be compared with a sensitive control of the same or similar species which is subjected to the same technical conditions of medium, incubation time, atmosphere, temperature and disc content. One may also ask, what is Kirby Bauer technique? Kirby-Bauer antibiotic testing (also called KB testing or disk diffusion antibiotic sensitivity testing) uses antibiotic-containing wafers or disks to test whether particular bacteria are susceptible to specific antibiotics. First, a pure culture of bacteria is isolated from the patient.What are the limitations of the disk diffusion assay?There are serious limitations to the use of disk diffusion method. Results may be unexpected or borderline. In such cases another method of testing may be required or the test may need to be repeated for confirmation.